http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-1808333-C
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_829316dbc94dd5651df97b32aaf2c1de |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-395 |
| filingDate | 1990-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1993-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c1761f89c9e3b6f6b5223aece6afb00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b3fd2671b1e636e411c187a1cf702e9e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_023f9297f4231926450b49c72b78b5ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f00195133e6f1823800ec9e1ed4ee1b0 |
| publicationDate | 1993-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-1808333-C |
| titleOfInvention | Method of oncoovarian acid-soluble alpha-2-globulin preparing |
| abstract | Usage: in medical biochemistry for the isolation of onco-ovarian acid-soluble alpha-2-globulin from tissues necessary for the development of specific immunodiagnostics. The purpose of the invention: obtaining a new tumor-associated acid-soluble antigen. SUMMARY OF GETTING: Tumor and metastatic tissue extract of primary ovarian adenocarcinoma is heated in a boiling water bath for 5-20 minutes at 95 ° C. The heated extract is treated with an equal volume of 5-15% trichloroacetic acid. The insoluble fraction is precipitated by centrifugation. An acid-soluble fraction is fractionated with ammonium sulfate. Dissolve the precipitate in a minimal volume of distilled water and gel filter it on a Sephadex G-100 column. Precipitated with ammonium sulfate fractions containing GAG. Dissolve the precipitate in distilled water, followed by gel filtration on a Sephadex G-200 column in volatile 0.05-0.2 M ammonium acetate buffer (pH 5.0-7.0} and fraction lyophilization containing the target product. Positive effect: the invention allows, under ordinary laboratory conditions, using available reagents to obtain a new onco-ovarian f / yal acid-soluble human protein. |
| priorityDate | 1990-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.