http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-1770359-C
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_592638d9e8f3946ee52e303f6335f38a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_48c7aa3f6e449434f2b41f4fbee4d60e |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate | 1988-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1992-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_39a4bf1fe0249eeb21b838f9343ebb68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1db90827d968d7e98f12efe7da1d887 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bfb2769db8394f0e47175a4adf0b849a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8f473fb3f3565c6c82701bbc9eb3aedc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b1656adcfe6bb8b4f927b2903f5137a7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8860177acf29d495970b0ac042de403f |
| publicationDate | 1992-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-1770359-C |
| titleOfInvention | Recombinant plasmid dna pj db (msil), encoding of human interleukin-2 synthesis in yeast cells saccharomyces cerevisiae, method of its preparation, and yeast strain saccharomyces cerevisiae - a producer of human interleukin-2 |
| abstract | The invention relates to biotechnology. The aim of the invention is to increase the yield of interleukin-2 in yeast cells by constructing recombinant pjDB plasmid DNA (MSIL), developing a method for constructing it and creating a strain transformed with this plasmid. The plasmid contains the yeast LEU 2 gene and a fragment of yeast two-micron DNA. ensuring its stable maintenance in yeast in a high number of copies; the coding region of the human interleukin-2 gene, flanked by the yeast PH05 gene transcription promoter and terminator, as well as bacterial DNA fragments that ensure plasmid replication in E. coli cells and carry the ampicillin resistance gene. The yeast cells of strain GC-1-GRF18 transformed with pjDB plasmid (MSIL) produce Inerleukin-2 in the order of 30-50 mg per liter of yeast culture, which corresponds to 10 units / ml. 3 s.p. f-ly. (L C |
| priorityDate | 1988-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 709.