http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-1692151-C
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_af6eb5bf72b8f7eac76ac4e5bc2811bf |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
| filingDate | 1990-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1995-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9fe041b50332b0c44fbd99e7361ce88a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52945bac0a58bc206a08e9fe7f70e98a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_210ad10aed286951c96af8f63e75cbcb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2f35d7e85047c09b0ca0f3ed28f243eb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a400bc2d230e32a3454086df229a6f6d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e379624cf34332270e6fe532ab44ea33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03fbe342fccf5506dc9e4875d5cfc5fb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8e87ce24c911206f526ea47711f0267f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8e8dc8d69687b79f7be917160233514d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_49da4ed154b75a94e2b4e8e68874f414 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_66daadd59f273216b57f35ba4f1c4653 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b2c6f0527ff7f3b4f3d1fadc94a5c8a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e07f087ef48aaac922e29861e1fa4c5e |
| publicationDate | 1995-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-1692151-C |
| titleOfInvention | Recombinant plasmid dna encoding plasminogen activator of urokinase type, method of its construction and the strain of bacterium escherichia coli - a producer of plasminogen activator of urokinase type |
| abstract | FIELD: genetic engineering. SUBSTANCE: modified activator encoding by plasmid pUABC differs from the natural urokinase by replacement of the first 24 amino acids of N-terminal domen (which is similar with epidermal growth factor) for 15 foreign amino acids. Plasmid pUABC is constructed by genetic-engineering methods on the basis of fragments of urokinase gene obtained after screeining of library cDNA. Expression of recombinant protein is carried out under control of regulatory elements of lac-operon of pUC 19 vector. Strain ВКПМ B-4534 a producer of modified plasminogen activator of urokinase type is prepared by transformation of E. coli jM 109 cells by plasmid pUABC. Strain ВКПМ B-4534 produces 2.2 g cell biomass per 1 l cultural fluid and produces modified activator mainly in proenzyme form (molecular mass is 43 kDa) at concentration 60000 units per 1 g biomass (1-1.5 mg per 1 l culture). Production of strain ВКПМ B-4534 does not depend on lac-operon expression inductor isopropylthiogalactopyranoside. Use of 2-mercaptoethanol enhances renaturation effectiveness by 5 times. EFFECT: preparing human modified plasminogen activator of urokinase type in proenzyme form without domen which is similar with growth factor, simplified method, decreased cost of bacteria growing. |
| priorityDate | 1990-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 177.