abstract |
The process according to the invention consists in that the peritoneal fluid collected from mice infested with the Toxoplasma gondii strain was washed three times by centrifugation at 500 rpm with physiological saline, then the combined supernatants were centrifuged at 1500 rpm / min for 30 min and the resulting sediment was resuspended in physiological saline, thermostated at 37 ° C for 1h with shaking, then centrifuged at 1500 rpm for 30 min, the resulting sediment was sonicated and then treat with sodium azide until a concentration of 0.5% is reached. |