| abstract |
The invention relates to a process for the preparation of an anti-tumor activity glycoprotein consisting of the extraction of the fruit bodies, myceliums or mycelium of a basidiomycete fungus belonging to the Coriolus group with hot water or aqueous alkaline solutions of a concentration of 0.1 ... 2.0 N at a temperature between 80 and 100 degrees C for 1-8h, neutralizing the extract thus obtained with an acid, the neutralized extract thus obtained is subjected to dialysis and / or ultrafiltration to remove substances with a lower molecular weight below 5000, the resulting substance is treated by a fractionated precipitation method at the isoelectric point at a pH of between 2.5 and 5.0 under pH conditions of the solution between 2.5 and 5.0 the ionic strength of the solution being 0.1-3.1 ° C at a temperature of between 5 and 25 ° C for more than 0.5 hours, the precipitate thus obtained being developed in the water followed by desalts drying and drying the solution thus treated. Basidiomycetes are selected from Coroiolus verzicolor ATTC 20547, Coriolus verzicolor ATCC 20548, Coriolus verzicolor ATCC 20549, Coriolus verzicolor ATCC 20549, Coriolus verzicolor ATCC 20550, Coriolus .................. |