http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RO-128665-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_21ff9fb4cdd8e76bbbc047458b574661 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 |
| filingDate | 2011-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8166ee519af04ba8a87aa953f6bf4be9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c6dfe93577e980b779e752539d12728 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40e6c14d9e41e15f1ac42ff96662f86b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4c0bae3d74c7b665953d5575a34ebb62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cec7ff535624320a2c5101dea0dad34d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8e00b4ff959838687af6fd7a7dcda7eb |
| publicationDate | 2017-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RO-128665-B1 |
| titleOfInvention | METHOD OF DETERMINING STEROIDIC HORMONES IN BIOLOGICAL MATRIX |
| abstract | The present invention relates to an analytic method for the specific highly-sensitive determination of steroid hormones in biological matrix, by solid-phase micro extraction, derivatization and detection. According to the invention, in a flask, provided with a septum and a magnetic stirrer, which contains the serum separated from blood, a polyacrylate fibre of 85 μ m is introduced through the septum, then NaCl is added for decreasing the estrogen solubility, the extraction is performed at 25...45°C for 100...120 min in thermostated bath, the fibre is withdrawn and the analytes are derivatized with N-methyl-N-trimethylsilyl-triflouro-acetamide and the silylated hormone derivatives are simultaneously determined by capillary gas chromatography coupled with mass spectroscopy. |
| priorityDate | 2011-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 24.