| abstract |
The invention relates to anprocess for obtaining a productnhepatoprotector, from beef spleen throughnthe successive treatment of the chloroformic extract,nobtained by autolysis, with acetone,npetrol decontamination, passage purificationnchromatographic column and treatment withnactive carbon, which consists in thatnconcentrated acetone extract and delipedntreated with 5% (w / v) phenol, purified withnactivated charcoal, chromatograph onnSephadex SPC 25 is eluted with waternsaturated chloroform and the biactive fractionnThe target is concentrated to 1/10 f A 'Arnvolume and treated with 1 ... 3% (w / v) brothn5% w / v phenol and 3n... 10% activated charcoal deprogenate, senfilter through a sterilizing membrane and itnlyophilize in a known manner. |