http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RO-108252-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5dfdc04a88424112a10b609494046b78 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P41-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07B57-00 |
| filingDate | 1990-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f4cd8608cc619aa5ac825a4c20bdb5d3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56d85c539755431b4a3a12dd87587982 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_81ad4322abda0a3b0897205fd24f52a2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6866b488d5c698040bc997a25c6f5955 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e98fd2f830c08c82823029b5ca7a8f6c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5be4dfaffe248b6ccaa82fcf82013092 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e08af726ec1e34751529d9a2eed3522 |
| publicationDate | 1994-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RO-108252-B1 |
| titleOfInvention | Enzymatic process for the separation of D - (-) - α-aminophenylacetic acid from racemic |
| abstract | The invention relates to a processnenzymatic separation of D - (-) - aminophenylacetic acidnof the racemic comprisingnthe following steps: - acetylation of D, L-aminophenylacetic acidnwith an excess of 20% molesnacetic anhydride in the presence of NaOH such as pHnto be slightly basic at a temperature of 5 ... 15 ° C, thennacidic with conc. up to /? H = 1 ... 2 whennprecipitates N-acetyl-D, L-α-aminophenylacetic acid,nwhich is filtered off and dried; - enzymatic hydrolysisnof the aqueous solution of N-acetyl-D, L-aminophenylacetic acidn0.1 M and pH = 7 containing 1 xnIO'4 M CoCl2 by passing the solution through ancolumn bioreactor with fungal aminoacylasenimmobilized on a modified dextran supportnchemically at a spatial velocity of 0.25 ... 0.35 h1 atn50 ° C, converting to L - (+) - aminoacetic acid;n- separation of L - (+) - aminoacetic acidnby concentrating the solutions obtained atnleaving the column, in vacuo to about 15 ° Cn... 20% of the initial volume, precipitation by coolingnat 15 ° C, filtration and drying; - separation of N-acetyl-D - (-) -? - aminophenylacetic acidnby acidificationnof the filtrate obtained with HC15N to pH = 1 ... 2,nwhen precipitating the same derivative that is filtered and evaporatedndries at moderate temperatures; - deacetylation bynheating the suspension of 30-35% by weight of N-acetyl-D - (-) -? - aminophenylacetic acid,nin 2.5N HClnreflux, for 3 ... 5h, cooling, correction of the aphnto 5.6 with 5 N NaOH, maintaining the suspensionnobtained at 4 ... 10 ° C for about 10 ...n14 h, followed by filtration and drying of the precipitate atnmoderate temperatures to yield D - (-) - aminophenylacetic acid;n- racemization of L - (+) - aminophenylacetic acidnconcentration of 5 to 10% bynrefluxing in 2N NaOH solution for 1 ... 3h,ncooling, adjusting the pH to 5.6 with 5N HCl,nmaintaining about 10 ... 14 hours at 4 ... 10 ° C,nfiltration and drying to yield D, L-α-aminophenylacetic acid,nwhich recirculates in the process at the stagenof acetylation. |
| priorityDate | 1990-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.