Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3e9445e5e3626f36e932c6451a928250 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-70535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6456 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-735 |
filingDate |
1987-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c67eb6d6a33c6fd833b301eb8a395ef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bfbcd6439e9889965f3c692c93175641 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b9d1eebe3ca0fd8437dbc95560eb0cea |
publicationDate |
1990-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
PT-86275-B |
titleOfInvention |
METHOD FOR THE PRODUCTION OF A RECOMBINANT DNA MOLECULE |
abstract |
Recombinant DNA molecules comprising a series of functional elements in the following order: (a) an enhancer/promoter unit linked operatively to (b) a DNA segment which can be transcribed into a mRNA having coding capacity for a desired protein product and comprising signals for polyadenylation and for efficient transportation to the cytoplasm, followed by a DNA segment (c) consisting a promoter unit (c₁) operatively linked to a DNA subsegment (c₂) which can be transcribed into the mRNA of a selection marker gene, wherein said promoter unit (c₁) lacks a functional enhancer but is capable of being stimulated by the enhancer located in (a) due to the absence of an enhancer stop DNA sequence between (a) and (c₁), are provided. The specific arrangement of the functional elements assures that a high proportion of transfected eukaryotic cells stably express the heterologous gene even when said DNA is applied to said eukaryotic cells as circular DNA. |
priorityDate |
1986-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |