http://rdf.ncbi.nlm.nih.gov/pubchem/patent/PL-212182-B1

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filingDate 2007-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_22e164aaeb150e6b8d7826c4ee8d20d3
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publicationDate 2012-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber PL-212182-B1
titleOfInvention A method for detecting and / or determining the enzymatic activity of lovastatin esterase using a fluoro / chromogenic substrate, a kit for detecting and / or determining the activity of lovastatin esterase and the use of a fluoro / chromogenic substrate for extracorporeal detection and / or determination of enzymatic activity
abstract The invention relates to a method for detection and/or assay of the lovastatin esterase enzymatic activity, with use of the fiuorogenic/chromogenic reagent, comprising use of the fiuorogenic/chromogenic reagent of formula II wherein R represents hydrogen or C1-C6-alkyl, R' represents one or two substituents of the ring A, R" represents one or two or three substituents of the ring B. According to a method, the reagent is added to a material of enzymatic activity and the hydrolysis reaction under enzyme action is carried out, followed by detection and/or assay of the liberated coumarin compound of formula III. Also the invention relates to lovastatin esterase isolated and/or purified using this method, an assembly for detection and/or assay of the lovastatin esterase enzymatic activity, an use of the fiuorogenic/chromogenic reagent of formula II for detection and/or assay of the lovastatin esterase enzymatic activity.
priorityDate 2007-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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