| abstract |
Method for the production of purified, expressed human prolactinnin eukaryotic cells, a recombinant DNA molecule encoding this protein is characterizednin that the fragment containing the mellitin sequence at the N terminus is attached to the DNA moleculenmolecule, the gene encoding human prolactin is expressed using baculovirusnthe expression system, and the externally secreted protein is precipitated by changing the pHnto 8.5 and then salted out with ammonium sulfate to 80% (w / v) concentrationnand purifies by affinity chromatography of the histidine amino acids attached tonpurified protein to zinc atoms bound to the solid phase in the chromatography column. |