http://rdf.ncbi.nlm.nih.gov/pubchem/patent/PL-165124-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b205247dfa6d7b3438398d81d6f7aa06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 |
| filingDate | 1990-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e73980109d12b1ceb22545bb30761a0b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_770bd632b581d03061b61fdd98f7aca3 |
| publicationDate | 1994-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | PL-165124-B1 |
| titleOfInvention | Method of obtaining intracellular glucose oxidase |
| abstract | Method for obtaining intracellular glucose oxidase from cultivated myceliumnby the deep method on a mineral medium enriched with the addition of peptone, characterized in thatnthe grown mycelium is washed with a buffer solution with a pH close to 7, preferablyncomposed of equal volumes of 0.1 M citric acid solution and 0.2 M phosphate solutionndisodium solution, and then the washed mycelium is treated with a solution similar to the one usednas a culture medium but with a lower glucose content, preferably with a compositionnby weight: 4% glucose, 0.038% diammonium phosphate (NH ^ HPO- :, 0.02 monopotassium phosphatenKH2PO4, 0.016% magnesium sulphate heptahydrate MgSO-t 7HzO, and withnaddition of 0.1% by weight of Tween 80 and 0.02% of sodium azide, the treatment of whichnspeech is carried out for 12-120 hours at 20-50 ° C, keeping the pH of the solution atnlimits 3-8 and preferably on a shaker or with agitation by aeration thereafternthe liquid is separated from the mycelium and processed further in a known manner. |
| priorityDate | 1990-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.