abstract |
A thermophilic anaerobic microorganism is cultured in the presence of cellulose and b-glucosidase, whereby a cellulase is produced to decompose the cellulose into a saccharide such as cellooligosaccharide, the resultant cellooligosaccharide is further decomposed with b-glucosidase into glucose and the glucose is accumulated in a culture medium. Alternatively, a thermophilic anaerobic microorganism is cultured in the presence of a mixture of starch and cellulose, b-glucosidase, a-amylase and glucoamylase, whereby a cellulase is produced to decompose the starch-cellulose mixture into glucose and the glucose is accumulated in a culture medium. |