http://rdf.ncbi.nlm.nih.gov/pubchem/patent/PE-20211109-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1d2090be81527e94a56d403dc02ece48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_de4c726463c613b653afe90e223a4817 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-02 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-00 |
filingDate | 2019-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d846dbb6c4273537fffbb6668cd5e22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8a003b1c25f71cc9f18c70d012f88c6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_113f057d3b53ea1b88da4f117dd9dbfd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58a21cf032d11b996c38ba067508ff82 |
publicationDate | 2021-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | PE-20211109-A1 |
titleOfInvention | CRYOPRESERVATION MEDIA SUPPLEMENTED WITH MACA |
abstract | The present invention relates to a process for the preparation of a cryopreservation medium, in particular one that is supplemented with maca to preserve tissue in the form of testicular fragments or biopsies; which comprises the following steps: a) Prepare a modified Ham's F10 medium (Ham's F10) which in 500 ml contains 4.9 g of Ham's F10, 0.0375 g of penicillin, 0.0375 g of streptomycin, 0.1225 g of calcium lactate, 2.73 g of Hepes, 0.168 g of sodium bicarbonate, and it is made up to 500 ml with ultrapure water, the obtained medium has pH 7.4; b) Supplement Ham's F10 medium modified with 10% dimethylsulfoxide (1.41 M) + 10% Fetal Bovine Serum + 200 mM of a sugar from the trehalose or sucrose group + 20 mg / mL of atomized extract of Black Maca; and c) Filtering the sterilized medium. |
priorityDate | 2019-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.