| abstract |
WHERE TO DETECT GOLD INCLUDES: a) OBTAIN A SAMPLE OF MENA THAT CONTAINS GOLD; b) CONTACTING A SURFACE OF THE SAMPLE WITH A SPECIFIC PROTEIN FOR GOLD; c) TO CONTACT SAID SURFACE WITH A PROTEOLYTIC AGENT TO PROTEOLIZE SAID PROTEIN IN A PROTEOLYTIC FRAGMENT; AND, d) DETECT SAID PROTEOLITIC FRAGMENT. THE SPECIFIC PROTEIN IS ONE OF HIGH SPECIFICATION AND AFFINITY FOR GOLD, THE PROTEOLYTIC AGENT IS TRYPSIN, AND THE PROTEOLYTIC FRAGMENT IS THE DOMAIN OF PLO ALKALINE PHOSPHATASE. EXTRACTING GOLD INCLUDES: i) OBTAINING A SAMPLE OF A PROCESSING SOLUTION CONTAINING GOLD AND MAGNETITE; ii) CONTACT THE SAMPLE WITH A REAGENT THAT BINDES THE MINERAL MAGNETITE, INCLUDING A SPECIFIC PROTEIN FOR GOLD TO FORM A COMPLEX OF MAGNETITE MINERAL BINDING REAGENT: GOLD; And, iii) APPLY A MAGNETIC FIELD TO THE SAMPLE. IF THE SAMPLE OF (i) CONTAINS ONLY GOLD, IT IS CONTACTED WITH A HYDROPHOBIC REAGENT INCLUDING A GOLD-SPECIFIC PROTEIN COMPLEX: GOLD, A FLOAT REAGENT IS ADDED TO THE SAMPLE, AND THE SAMPLE IS SHAKED |