| abstract |
Disclosed is Lactoferrin having more than 95% purity, substantially free of LPS, endotoxins and angiogenin with an iron saturation level comprised between 9%-20%. Also disclosed is a method for the production of said lactoferrin. The method comprising at least the steps of: disposing of raw material selected in the group consisting of skimmed milk before the pasteurization, caseinate-free milk, cheese-whey or skimmed colostrum that has not been treated at a temperature greater than 50 degrees Celsius; submitting this raw material to a treatment in order to obtain a solution of lactenin (LN) or Milk Basic Protein (MBP); submitting the LN or MBP solution to a purification step on a cation exchange resin equilibrated with an acetate buffer at a pH between 4 and 9 and eluted with different buffer solutions containing different solute concentrations; and collecting a fraction containing lactoferrin having more than 95% of purity, having no polymers and substantially free of LPS, endotoxins and angiogenin. |