http://rdf.ncbi.nlm.nih.gov/pubchem/patent/NZ-581699-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_036e0569529cd8261e371c018f935407 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-532 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-77 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-532 |
| filingDate | 2008-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a069d0b35d16136e0ed0c3ad5d27ebcf |
| publicationDate | 2012-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | NZ-581699-A |
| titleOfInvention | A method for preparing labelled molecule conjugates utilising a scaffold molecule, a small molecule ligand and a thiol generator |
| abstract | Disclosed is a method for indirectly coupling a small molecule ligand to a molecule to be labelled with the ligand, the method comprising the steps of: attaching at least one small molecule ligand which incorporates an N-hydroxy succinimide derivative to a scaffold molecule (such as dextran derivatives); and subsequently contacting, in a single mixture, the scaffold molecule (with the attached at least one small molecule ligand); the molecule to be labelled (which is an enzyme, an antibody or an antigen-binding fragment or variant of an antibody etc.); and a thiol-generator (which is typically 2-iminothiolane (also known as Traut's reagent); wherein the thiol-generator forms thiol groups in situ on the molecule to be labelled; the scaffold molecule having at least one group which is reactive towards the thiol group formed on the molecule to be labelled, so as to form a bond between the scaffold molecule and the molecule to be labelled, thereby indirectly coupling the small molecule ligand to the molecule to be labelled; and wherein the small molecule ligand is typically selected from the group consisting of: fluorophores; chromophores; biotin; avidin; metal ion chelators; photoreactive groups; iodinatable moieties; photosensitisers; quenchers; peptides; and low molecular weight drugs. |
| priorityDate | 2007-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 24.