http://rdf.ncbi.nlm.nih.gov/pubchem/patent/NZ-576113-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5610690048edbc271e03b26b23f76ff9 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2561-109 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2523-101 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2008-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93d98cd33cbd15a300da6fd9dd079428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a3f27cb4b876e6e6c26fef106722c6d |
| publicationDate | 2012-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | NZ-576113-A |
| titleOfInvention | Microorganism detection method and microorganism detection kit |
| abstract | Disclosed is a method of detecting a live cell of a microorganism by distinguishing live cells from dead cells and injured cells of the microorganism in a test sample comprising the steps of: a) adding cross-linker capable of binding covalently to chromosomal DNA or intercalating into chromosomal DNA by irradiation with light having a wavelength of 350 nm to 700 nm to the test sample; b) irradiating the test sample to which the cross-linker is added with light having a wavelength of 350 nm to 700 nm; c) removing the cross-linker contained in the test sample irradiated with light; d) adding a medium to the test sample from which the cross-linker is removed and incubating the test sample; e) adding again the cross-linker capable of cross-linking a DNA by irradiation with light having a wavelength of 350 nm to 700 nm to the incubated test sample; f) irradiating the test sample to which the cross-linker is added with light having a wavelength of 350 nm to 700 nm; g) extracting a DNA from the test sample and amplifying a target region of the extracted DNA by a nucleic acid amplification method; and h) analyzing the amplified product based on a standard curve which is created by using a standard sample of the microorganism and shows a relationship between an amount of the microorganism and the amplified product. Also disclosed is a kit when used according to the said method, comprising the following components: cross-linker capable of binding covalently to chromosomal DNA or intercalcating into chromosomal DNA by irradiation with light having a wavelength of 350 nm to 700 nm; a medium; and one or more primers for amplifying a target region of a DNA of a microorganism, which is a detection target, by the nucleic acid amplification method. |
| priorityDate | 2007-08-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 139.