| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_69ece09bafee7e0c49d1f7951a3faa2c |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-52 |
| filingDate |
2006-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b03e739bbe7193b18a0acec115ef81bf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_228b8808854953cb7d15c158aa4a8159
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c661364a499b3f805b91b0b8cc7db1b4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_acc24774b8a972c5156e7eb6a8ab1445
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c1996dff1348e504e6e89a486819d531
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62b614ead29d2f7121e3a0adbf77671e |
| publicationDate |
2011-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
NZ-568809-A |
| titleOfInvention |
Recovering and purification of VEGF proteins from prokaryotic cells using polyanionic agents |
| abstract |
A process for recovering the heparin binding protein, vascular endothelial growth factor (VEGF), from a prokaryotic cell culture. The process comprises the steps: (a) isolating the VEGF from the periplasm of the prokaryotic cell culture; (b) denaturing the VEGF in a first buffered solution that comprises a chaotropic agent and reducing agent; (c) incubating the denatured VEGF in a second buffer solution that comprises a chaotropic agent and a sulphated polyanionic agent for a time sufficient to refold the VEGF; (d) recovering the refolded VEGF, wherein there is a 2-5 fold increase in refolded VEGF compared to incubating with no sulphated polyanionic agent. The polyanionic agent can be dextran sulphate or sodium sulphate and the VEGF can be VEGF165. |
| priorityDate |
2005-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |