abstract |
Provided is an RNAi expression cassette for targeting multiple regions of a HCV genome, comprising: a promoter; an RNAi agent with at least two coding sequences, and sequences coding for spacer regions; wherein transcription of the RNAi agent coding sequences produces RNAi agents which form stem-loop structures, the stem loop structures being spaced from one another by the spacer regions. The compositions can be used to treat HCV infections. |