Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_69ece09bafee7e0c49d1f7951a3faa2c |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-92 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-55 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-705 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-70535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-70596 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-245 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2887 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2896 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-705 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
filingDate |
2005-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_855faa746d11a7c6bc7991238f7079c4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb8e10d45903adc82d9471d81b30ac7e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_26ca3bfe9e1f253bc5473427179c8089 |
publicationDate |
2010-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
NZ-556635-A |
titleOfInvention |
Method for producing soluble multi-membrane-spanning proteins |
abstract |
Disclosed is an expression construct for expressing a single or multiple membrane spanning polypeptide in a host cell, the expression construct comprising: a) a polynucleotide encoding the single or multiple-membrane-spanning polypeptide; b) a tightly controlled promoter having low basal activity in the host cell, and having at least one positive regulatory control element and at least one negative regulatory control element, wherein one or more of the positive or negative regulatory control elements is a heterologous regulatory control element; and c) a leader sequence comprising a translation initiation enhancer. Also disclosed is a method for producing a membrane spanning polypeptide using the expression construct and polypeptides produced by said method. |
priorityDate |
2004-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |