| abstract |
Provided is a method of producing a variant glycolipid acyltransferase enzyme comprising selecting a parent enzyme which is a lipid acyltransferase enzyme wherein the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S and modifying one or more amino acids to produce a variant lipid acyltransferase, aided by structural homology mapping and inclusion of specified amino acid changes compared to wildtype sequence. Further provided are methods of using the enzymes in food processing, e.g. enzymatic degumming of vegetables of edible oils. |