Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9a95d0c63b9b3f2bc8e8d6b45fbe7789 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-21 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-1282 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-13 |
filingDate |
2004-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_88b319d72b609609f7d1bd69d419f8f7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_120bd45982ed51ca87512f2076ceebc3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_17cb746bfd9fdb4e754517d618e375c0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_be0fcf07629c6a66fa29c7217d3def68 |
publicationDate |
2009-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
NZ-545936-A |
titleOfInvention |
Method for linking variable region encoding sequences using multiplex molecular amplification using a template derived from a single cell |
abstract |
Disclosed is a method for linking a plurality of non-contiguous nucleotide sequences of interest comprising: a) amplifying, in a multiplex molecular amplification procedure, nucleotide sequences that comprise variable regioin encoding sequencesusing a template derived from an isolated single cell; and b) effecting linkage of the amplified sequences to form a cognate pair of variable encoding sequences. The disclosure provides an efficient method of linking two or more nucleotide sequences encoding for domains or subunits of a heteromeric protein, in a single reaction. Especially, the linkage of variable region encoding sequences from e.g. immunoglobulins, T cell receptors or B cell receptors is eased. The disclosed method allows for a more efficient way of generating libraries of variable region encoding sequences. The capability to perform the multiplex overlap-extension RT-PCR using template derived from an isolated single cell enables the generation of cognate pair libraries in a high-throughput format. |
priorityDate |
2003-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |