| abstract |
Disclosed is an in vitro method for producing a biologically active Neublastin polypeptide, comprising culturing a cell comprising an expression vector comprising a nucleic acid comprising a promoter sequence operably linked to a nucleotide sequence encoding a signal peptide and a neublastin polypeptide, wherein said nucleotide sequence does not encode a neublastin pro-region. and wherein said signal peptide is an immunoglobulin signal peptide, a TGF-beta signal peptide, a GDF signal peptide, an IGF signal peptide, a BMP signal peptide, a Neurotrophin signal peptide, a PDGF signal peptide, an EGF signal peptide, an insulin signal peptide, an ADH signal peptide, an LH signal peptide, an FSH signal peptide, an ACTH signal peptide, an MSH signal peptide, a TSH signal peptide, a DHEA signal peptide, an interleukin signal peptide, a neurturin signal peptide, a GDNF signal peptide, a persephin signal peptide, an NGF signal peptide, a signal peptide being the signal peptide contained in SEQ ID NO:70, or a native Neublastin signal peptide. |