| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d7821e4854d2ca5e475d3dbc3bc5ff9d |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-19 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate |
2004-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6b2840963d569bb49b453a9bb7d77e55
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b3300b53888bd168441d84858d43e61
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f5bb94a29741276f41a78b757b7d2d9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec79f176a6d21c755eb24aae6c1af821
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5329fa295732289bebaa0409718cfdbb |
| publicationDate |
2007-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
NZ-541192-A |
| titleOfInvention |
Plasmid-free clone of E. coli strain DSM 6601 and method for its creation using sacB and two resistance genes introduced into the pMut1 and pMut2 plasmids |
| abstract |
Disclosed is a plasmid free clone of the E. coli strain DSM 6601, and a method of preparing it. The method involves the introduction of a resistance gene and the sacB gene into each of the plasmids pMut1 and pMut2, which are the plasmids that are usually found in this strain. The modified plasmids are introduced into the bacteria under conditions such that the naturally occurring plasmids are replaced by the modified ones. The bacteria are grown under conditions selecting for the particular resistance used in the plasmids, and then are grown under conditions that select against sacB. Preferably each plasmid has a different antibiotic resistance, and more preferably the pMut1 plasmid has a tetracycline resistance cassette, and pMut2 plasmid has a kanamycin resistance cassette, with the elimination of each plasmid taking place in two separate steps. |
| priorityDate |
2003-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |