http://rdf.ncbi.nlm.nih.gov/pubchem/patent/NZ-538608-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ff3e741d8c3082a79ba472a5a4c2bef8 |
| filingDate | 2002-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c73b92c6a3d8c6f422de1b247448909 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4903a12cd99a69496919bd211597d418 |
| publicationDate | 2006-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | NZ-538608-A |
| titleOfInvention | Detecting cytosine methylation in genomic DNA samples in which all unmethylated cytosine bases are converted to uracil and the 5-methylcytosine bases remain unchanged |
| abstract | A method for the detection of cytosine methylation in DNA samples is described, characterized in that the following steps are conducted: a genomic DNA sample, which comprises the DNA to be investigated as well as background DNA is chemically treated in such a way that all of the unmethylated cytosine bases are converted to uracil, while the 5-methylcytosine bases remain unchanged; the chemically treated DNA sample is amplified with the use of at least two primer oligonucleotides as well as a polymerase, whereby the DNA to be investigated is preferred over the background DNA as the template, and whereby the amplification is not conducted in the presence of additional oligonucleotides or a PNA oligomers, which preferably bind to 5'-CG-3' dinucleotides or a 5'-TG-3' dinucleotides or 5'-CA-3' dinucleotides of the background DNA and adversely affect its amplification, and the amplified products are analyzed and the methylation status in the DNA to be investigated is concluded from analysis of additional positions. |
| priorityDate | 2001-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.