| abstract |
Disclosed is a bacterium in whose genome the following components are inserted and can be expressed: I) a nucleotide sequence that encodes a directly or indirectly, antiproliferatively or cytotoxically active expression product or a plurality of said expression products, II) a nucleotide sequence that encodes for a blood plasma protein under the control of an activation sequence that can be activated in the bacterium, or that is constitutively active, III) optionally, a nucleotide sequence that encodes for a cell-specific ligand under the control of an activation sequence that can be activated in the bacterium, or is constitutively active, IV) a nucleotide sequence for a transport system that induces expression of the expression products of components I) and II) and optionally III) on the outer surface of the bacterium or that induces secretion of the expression products of component I) and expression of component II) and optionally III) and that is optionally constitutively active, V) optionally a nucleotide sequence for a protein for lysis of the bacterium in the cytosol of mammalian cells and for the intracellular release of plasmids contained in the lysed bacterium, and VI) an activation sequence that can be activated in the bacterium, and/or that is tissue cell-specific, tumor cell-specific, function-specific or not cell-specific, for expressing component I), wherein any of components I) to VI) is present either once or several times, and either identical or different. |