abstract |
A polypeptide conjugate exhibiting G-CSF activity, comprising a polypeptide with an amino acid sequence that differs in 2-10 amino residues from the amino acid sequence of SEQ ID NO: 1. The polypeptide comprises at least one substitution selected from K16R/Q, K34R/Q and K40R/Q and at least one substitution selected from T105K and S159K relative to SEQ ID NO: 1. The conjugate has 2-6 polyethylene glycol moieties with a molecular weight of about 1000-10,000 Da attached to attachment groups of the polypeptide. |