Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dea97595058f316a875c28a64efb90da |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2549-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-525 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-186 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848 |
filingDate |
2002-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0ed6cf92586d757b86b04cdb87a7001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1039bd4fe0e32a9a82aeb070d76d26fd |
publicationDate |
2005-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
NZ-528919-A |
titleOfInvention |
Amplification process using pyrophosphate |
abstract |
A method for conducting a nucleic acid amplification reaction, said method comprising forming an amplification reaction mixture in the presence of sufficient of a pyrophosphate salt to prevent primer extension taking place, digesting said pyrophosphate salt with a pyrophosphatase enzyme (PPase), and subjecting said reaction mixture to conditions such that an amplification reaction may proceed. This can be used as a "hot start" amplification. Particular novel pyrophosphatase enzymes for use in the method are also described and claimed. |
priorityDate |
2001-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |