| abstract |
Disclosed is a method of producing a heterologous peptide, polypeptide or protein in a lactic acid bacterium, the method comprising: (i) constructing a recombinant lactic acid bacterium comprising a nucleotide sequence coding for the heterologous peptide, polypeptide or protein and operably linked thereto, appropriate regulatory nucleotide sequences to control the expression of the coding sequence, (ii) cultivating the recombinant bacterium under fed-batch or continuous cultivation conditions in a chemically defined medium to express the gene, and (iii) harvesting the recombinant bacterium or the peptide, polypeptide or protein. Also disclosed is a chemically defined basal medium (LM1 medium) for cultivating bacteria. |