abstract |
A method of preparing a composition comprising a mixture of cells derived from human liver tissue, which mixture comprises an enriched population of human liver progenitors, comprising: a) providing a cell suspension of human liver tissue comprising a mixture of cells of varying sizes, including immature cells and mature cells; and b) debulking the suspension based on cell size, buoyant density, or a combination thereof to remove mature cells, while retaining immature cells. Also covered is the use of human liver progenitors isolated by this method in the preparation of a medicament for treating liver dysfunction. |