http://rdf.ncbi.nlm.nih.gov/pubchem/patent/NZ-504473-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b37014766a57d712bba498b84d48e0ef |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-70503 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8258 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-705 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18 |
| filingDate | 1998-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_448ffb382c6e1337166cbfedfc8313e2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61611d86da12269bd1a863c5addb29b0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_39766ce59ec13807380f9257a5921766 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03d9b6cb7009c0452ace5af01bb5ecd5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99cd9adaaba6868b65e1b2f91a897e7f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d9724602fa84f9ad461cf0a8d6988e6 |
| publicationDate | 2001-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | NZ-504473-A |
| titleOfInvention | Multimeric proteins within transgenic alfalfa plants and their use in diagnostic assays |
| abstract | The characterising of a plant production system suitable for transgenic proteins that meet the stringent U.S. Government regulatory requirements is described. The production and characterisation of an anti-human IgG for use as a blood grouping reagents is exemplified through the expression of corresponding genes in transgenic alfalfa plants. The cDNAs of the heavy and light chains of a human IgG-specific IgG2a(kappa) murine mAb (C5-1) were transferred into alfalfa through Agrobacterium infection. Transgenic plants expressing the light- and heavy-chains encoding mRNAs were obtained and plants from the F1 progeny (obtained by sexual crossing) were found to express fully assembled C5-1. Furthermore, the transgenic protein was stable in vivo, as well as during extraction and purification procedures. Purification yielded a unique H2L2 form with reactivity indistinguishable from hybridoma-derived C5-1 in standardised serological tests. Results indicate that plant-derived transgenic proteins, such as mAbs can be used as diagnostic reagents as effectively as hybridoma-derived mAbs, and demonstrates the usefulness of the transformed alfalfa system to produce large amounts of proteins, including multimeric proteins such as mAbs. |
| priorityDate | 1997-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.