http://rdf.ncbi.nlm.nih.gov/pubchem/patent/NZ-337900-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3120ae9cab5bb4831f0cc16288966367 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6811 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 |
| filingDate | 1998-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f219cb72438615eb929f0765b67a4a0 |
| publicationDate | 2001-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | NZ-337900-A |
| titleOfInvention | DNA shuffling for randomised heterologous and homogenous 3' and 5' primers for construction of DNA libraries for generation of more desirable mutants |
| abstract | A method of shuffling polynucleotides comprising: (a) providing an isolated sense strand from at least a first variant form of a polynucleotides, and an isolated antisense strand from at least a second variant form of the polynucleotides, wherein the isolated sense and antisense strands are starting substrates to be shuffled; and (b) shuffling the isolated sense and antisense strands provided in step (a) by contacting the strands with at least one primer or one pair of primers and conducting a multicyclic polynucleotides extension reaction wherein (i) in at least one cycle, the primer(s) anneal to the isolated strands and generate extended primers; and (ii) in at least one subsequent cycle, the extended primers generated in a previous cycle act as both primers and templates to form further extended primers, whereby the polynucleotides extension is continued for sufficient cycles until the further extended primers includes recombinant forms of the shuffled polynucleotides. The method enables optimised DNA sequences to be obtained in order to (a) enhance the properties of a protein of interest by artificial generation of genetic diversity by creating a library of "genes", which may be screened for specific characteristics. |
| priorityDate | 1997-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 534.