http://rdf.ncbi.nlm.nih.gov/pubchem/patent/NL-2028946-B1

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filingDate 2021-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d6f202512568eee9cd9ddd0ac8f419f3
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publicationDate 2022-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber NL-2028946-B1
titleOfInvention Cryoprotective solution and ultra-low temperature cryopreservation method of stichopus japonicus sperm
abstract The invention relates to the technical field of ultra-low temperature cryopreservati on of stichopus japonicus germplasm, in particular to a cryoprotective solution, an activator 5 formula and an ultra-low temperature cryopreservation method of apostichopu japonicus sperm based on a program controlled freezer and CASA. The cryoprotective solution is composed of a diluent, an antifreeze, and an additive, wherein the diluent is natural seawater, the antifreeze is one or more of aprotic polar solvents, and the additive is glucose. The method of the present invention has a large amount of frozen storage (more 10 than 200 mL of fresh stichopus japonicus semen can be cryopreserved in one batch), can precisely control the cooling process, and has a good application effect after thawing. It can solve the problem of unsynchronized maturation of male and female sea cucumbers and a series of germplasm degradation of stichopus japonicus in actual production.
priorityDate 2020-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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