http://rdf.ncbi.nlm.nih.gov/pubchem/patent/NL-2028946-B1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9c8c98a38f38e8f0ca609b2b6540a82e |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0284 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0221 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0226 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-021 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 |
filingDate | 2021-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d6f202512568eee9cd9ddd0ac8f419f3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_172efc85481c07bc4b1620fa99191fcb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7db94091eba64cf434f77ed872fadc05 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_413da83d7e0d59fe0664a7ce7cdb20ff http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7648614d7bd5a2dc4cd67e1333b6c659 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28eb8dd7c4d13a129646e8aff091158e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_727eda54f2a40cac77dc0a3f8532688c |
publicationDate | 2022-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | NL-2028946-B1 |
titleOfInvention | Cryoprotective solution and ultra-low temperature cryopreservation method of stichopus japonicus sperm |
abstract | The invention relates to the technical field of ultra-low temperature cryopreservati on of stichopus japonicus germplasm, in particular to a cryoprotective solution, an activator 5 formula and an ultra-low temperature cryopreservation method of apostichopu japonicus sperm based on a program controlled freezer and CASA. The cryoprotective solution is composed of a diluent, an antifreeze, and an additive, wherein the diluent is natural seawater, the antifreeze is one or more of aprotic polar solvents, and the additive is glucose. The method of the present invention has a large amount of frozen storage (more 10 than 200 mL of fresh stichopus japonicus semen can be cryopreserved in one batch), can precisely control the cooling process, and has a good application effect after thawing. It can solve the problem of unsynchronized maturation of male and female sea cucumbers and a series of germplasm degradation of stichopus japonicus in actual production. |
priorityDate | 2020-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.