http://rdf.ncbi.nlm.nih.gov/pubchem/patent/NL-2022391-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_08dde60e3e0ca1e6ffaa5143e5c8477a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4b10aa964c21a57e6056fb836049ca02 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6818 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-10 |
| filingDate | 2019-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a864bee9b570649e1059b42b4647360 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7adfc4a477ae6804cb3af35588f71610 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3a6d2ede31d3dd58534cb28d6c2d539 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d3cf197ab0b12d88e553de24d960928 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83d02675e43c20ba2f73883a84319515 |
| publicationDate | 2020-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | NL-2022391-B1 |
| titleOfInvention | An identification method of peptides binding with mhc i and their anchor residues |
| abstract | The disclosure provides an identification method of peptides binding with MHC I and their anchor residues by a random peptide library, including the following steps: (1) synthesizing random peptides with random sequence lengths of 8-12, (2) performing De Novo analysis on the random peptides, to obtain an amino acid distribution; (3) expressing the d chains and [32m chains of MHC I, (4) forming MHC I-peptide complexes from the random peptides, d chains and [32m chains in solution; (5) performing thermal denaturation, separation and purification on the MHC I-peptide complexes to obtain the peptides binding with MHC I, (6) performing De Novo analysis on the peptides binding with MHC I, to obtain an amino acid distribution of the peptides binding with MHC I, which was compared with the amino acid distribution in step (2) to give the major anchor positions and preferable anchor residues. The present method is simple to operate, has reliable results, takes short time, and achieves a high effective screening of potential pathogen-specific T cell epitopes, which could be extensively applied in preliminary screening of peptide vaccines for animals and human. |
| priorityDate | 2018-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 85.