| abstract |
A REAGENT IS SUITABLE FOR MEASURING THE CONCENTRATION OF AN ANALYTE IN A HEMOGLOBIN-CONTAINING BIOLOGICAL FLUID, SUCH AS WHOLE BLOOD. THE REAGENT COMPRISES A FLAVIN-DEPENDENT ENZYME THAT HAS SPECIFICITY FOR THE ANALYTE, A FLAVIN COFACTOR IF, AND ONLY IF, A FLAVIN IS NOT BOUND TO THE ENZYME, A TETRAZOLIUM DYE PRECURSOR, AN ELECTRON TRANSFER AGENT, AND A NITRITE SALT. THE REAGENT CAUSES DYE FORMATION THAT IS A MEASURE OF THE ANALYTE CONCENTRATION. THE NITRITE SALT SUPPRESSES INTERFERING DYE FORMATION CAUSED NON-ENZYMATICALLY BY RTHE HEMOGLOBIN. PREFERABLY, THE REAGENT IS USED IN DRY STRIP FOR MEASURING GLUCOSE IN WHOLE BLOOD.(FIG 1) |