| abstract |
THE PRESENT INVENTION RELATES TO TRANSFER PRIMARY, SECONDARY AND IMMORTALIZED CELLS OF VERTEBRATE ORIGIN TRANSFECTED WITH EXOGENOUS DNA WHICH ENCODES A THEREPEUTIC PRODUCT OR IS ITSELF A THEREPEUTIC PRODUCT, METHODS BY WHICH PRIMARY, SECONDARY AND IMMORTALIZED CELLS ARE TRANSFECTED TO INCLUDE EXOGENOUS DNA, INCLUDING DNA TARGETING BY HOMOLOGOUS RECOMBINATION, METHODS OF PRODUCING CLONAL OR HETEROGENOUS CELL STRAINS, AND METHODS OF GENE THERAPY.THE PRESENT INVENTION INCLUDES PRIMARY AND SECONDARYSOMATIC CELLS AND SOMATIC CELL PRECURSORS, ANDIMMORTALIZED CELLS THAT ARE CAPABLE OF DNA AMPLIFICATION, WHICH HAVE EXOGENOUS DNA STABLY INTEGRATED INTO THEIR GENIMES OR EXOGENOUS DNA PRESENT IN THE FORM OF AN EPISOME.(FIG. 11) |