| abstract |
The present invention relates to the production of polyketides and other natural products, and libraries of compounds and new individual compounds. An important area is the isolation and potential use of the new analogues of FKBP ligands and the host cells that produce these compounds. The invention is particularly related to the methods for efficient transformation of strains that produce analogs of FKBP, and recombinant cells in which the donated genes or gene cassettes are expressed, to generate new compounds such as ligand analogs. of FKBP polyketides (especially rapamycin), and the processes for their preparation, and the means used herein (for example, nucleic acids, vectors, gene cassettes and genetically modified strains). |