| abstract |
Provided herein are methods for efficient in vitro differentiation of HLA-homozygous blood cell derived pluripotent stem cells to hematopoietic precursor cells, and further differentiation of hematopoietic precursor cells into HLA-homozygous immune cells of various myeloid or lymphoid lines, particularly lymphocytes. T, NK cells and dendritic cells. Pluripotent cells can be maintained and differentiated under defined conditions; the use of mouse or serum feeder cells is not required in certain modalities for the differentiation of hemtopoietic precursor cells. |