| abstract |
The generation of H1N1 influenza HA polypeptides optimized to induce a broadly reactive immune response to H1N1 influenza virus isolates is described herein. Optimized HA polypeptides were developed through a series of HA protein alignments, and subsequent generation of consensus sequences, based on the selected H1N1 viruses isolated from 1918-2012. Optimized H1N1 HA polypeptides, and compositions, fusion proteins and VLPs comprising HA polypeptides are provided herein. In addition, codon optimized nucleic acid sequences encoding HA polypeptides are provided. Methods for inducing an immune response against influenza virus in a subject are also provided by the present disclosure. |