| abstract |
A genetically modified mouse is provided, wherein the mouse expresses an immunoglobulin light chain repertoire characterized by a limited number of variable light chain domains. Mice that express only one or some immunoglobulin light chain variable domains of a limited repertoire in their germ line are provided. Methods for making bispecific antibodies having universal light chains are provided using the mice described herein, which include variable regions of human light chain. Methods are provided for making human variable regions suitable for use in multispecific binding proteins, for example, bispecific antibodies, and host cells. Bispecific antibodies capable of binding a first and second antigen are provided, wherein the first and second antigens are separate epitopes of a single protein or epitopes separated into two different proteins. |