abstract |
The present invention relates to a method for producing carbamoyl phosphate, the method comprises reacting ammonia, ATP, bicarbonate and CO2, or a hydrated form thereof, in a composition in the presence of a carbamate kinase, wherein the ammonia and CO2 , or hydrated form thereof, are converted to carbamate in a chemical reaction and carbamate and ATP are converted to carbamoyl phosphate in an enzyme catalyzed reaction by carbamate kinase, and wherein the pH of the composition is about 8 to about 12 The invention also relates to methods for producing urea. |