| abstract |
The present invention relates to a random-loop biosynthetic polypeptide or a segment of the biosynthetic random or biosynthetic conjugate biosynthetic polypeptide, wherein the random-spiral biosynthetic polypeptide, the segment of the random-spiral biosynthetic polypeptide or the biosynthetic conjugate comprises a sequence of amino acids consisting solely of amino acid residues of proline and alanine, where the amino acid sequence consists of at least about 50 amino acid residues of proline (Pro) and alanine (Ala). At least about 50 amino acid residues of proline (Pro) and alanine (Ala) may be the constituents of a heterologous polypeptide or a construct of a heterologous polypeptide. The uses and methods of use of these biosynthetic random-polypeptides or the polypeptide or conjugate segments are also described. The uses may, inter alia, include medical uses, diagnostic uses or uses in the food industry, as well as other industrial applications, such as in the paper industry, in oil recovery, etc. In particular, a conjugate of a drug is provided which comprises (i) a polypeptide or the segment of the biosynthetic random spire polypeptide, which comprises an amino acid sequence consisting solely of amino acid residues of proline and alanine, wherein the sequence of amino acids consists of at least 50 amino acid residues of proline (Pro) and alanine (Ala), and (ii) a drug selected from the group consisting of (a) a biologically active protein or polypeptide, which comprises or is a sequence of amino acids that have or average a biological activity and (b) a small molecule drug. In addition, the nucleic acid molecules encoding the polypeptide or segment of the randomly spiked biosynthetic polypeptide and / or the biologically active heterologous proteins, as well as the vectors and cells comprising the nucleic acid molecules, are described. In addition, the present invention provides compositions comprising the compounds of the invention, as well as the specific uses of the polypeptide or random polypeptide segment, biologically active proteins, drug conjugates or nucleic acid molecules, vectors and cells. of the invention. Methods are also provided for producing and / or obtaining the inventive random spire biosynthetic polypeptides or segments, as well as for producing and / or obtaining biologically active, inventive heterologous proteins and / or polypeptide or conjugate constructs, such as drug conjugates. |