| abstract |
The present invention describes a method for purifying an immunoglobulin, wherein the method comprises applying an aqueous, pH-regulated solution comprising an immunoglobulin in monomeric form, aggregated, and fragmented to an anion exchange chromatographic material under conditions in which the Immunoglobulin in monomeric form does not bind to the anion exchange material, and immunoglobulin is recovered in monomeric form in the flow of the chromatographic anion exchange material, while the pH-regulated solution has a pH value of 8.0 to 8.5 . In one embodiment the anion exchange chromatographic material is a membrane anion exchange chromatographic material. |