| abstract |
Compositions comprising a cocaine esterase (CocE) and a compound that thermostabilizes CocE are provided. Methods for thermostabilizing a cocaine esterase were also provided. Additionally methods are provided for treating mammals under cocaine-induced condition. Methods to determine if a compound is a thermosetting agent for a protein are also provided. The uses of the compositions described above for the treatment of a cocaine-induced condition are further provided. In addition, an isolated nucleotide acid encoding a CocE polyopeptide having the substitutions L169K and G173Q, and the CocE polyopeptide encoded by such nucleic acid, and pharmaceutical compositions thereof, are provided. The use of such a composition is also provided for the manufacture of a medicament for the treatment of a cocaine-induced condition and for the treatment of a cocaine-induced condition. |