http://rdf.ncbi.nlm.nih.gov/pubchem/patent/MX-2009014098-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bb1f5a047939f83cce008b654f7fdc8c |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 |
| filingDate | 2009-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3f4d0c4d87a758150d72c6d49d7fc71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3f182bf586e389ae00350f4de64583d7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19087f684bb938deb8f3af1bfc99b924 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_efdb3952ee3447495cb968a79498eb63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_411591c469f11c260ba4fc37cbbf17f1 |
| publicationDate | 2011-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | MX-2009014098-A |
| titleOfInvention | IMMUNO-TEST TO DETERMINE THE AMOUNT OF SPECIFIC ANTIBODIES AGAINST INFLUENZA VIRUSES A / H1N1 / 2009 IN SAMPLES OF SERUM AND BLOOD PLASMA. |
| abstract | The standard method for the quantification of anti-influenza antibodies, the method of hemagglutination inhibition, requires the use of erythrocytes and viral particles. This makes it difficult to implement the method. The invention is presented of an immunological method (ELISA type) for the diagnosis of the presence, concentration or titre of anti-Influenza A / H1N1 / 2009 antibodies in blood serum or plasma samples of human subjects or animal models such as ferrets, rats, mice, among others. The proposed method does not require the use of viral particles or viruses. The fundamental component of the diagnostic method is a protein, referred to in the invention as HA50-274-h1n1. Said protein is a fragment of the H1n1 / 2009 Influenza A virus Hemagglutinin protein expressed in Escherichia coli strains. In its recommended version, the diagnostic method of the present invention comprises fixing an anti-histidine antibody to the surface of the diagnostic device; blocking of the remaining spaces of the surface with a protein or other blocking agent; the addition of the HA50-274-H1N1 protein; the addition of a test serum sample diluted in PBS solution at 1:50 ratio; the addition of a peroxidated bound anti-IgG antibody; the addition of substrate for the peroxidation reaction; the evaluation of the degree of reaction occurrence by absorbance reading at 580 nm. This method can be used for the evaluation of anti-Influenza A / H1N1 antibody titres in applications related to the development of influenza vaccines, in serological-based epidemiological studies, in studies for the determination of susceptibility to Influenza A / H1N1, etc. The method is particularly useful for the evaluation of samples taken from the third week post-infection. |
| priorityDate | 2009-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 228.