| abstract |
The present invention relates to molecules and a method in which one or more biologically active peptides without incorporated into an Fc domain. In this invention, pharmacologically active compounds may be prepared by a method comprising (a) selecting at least one peptide that modulates the activity of a protein of interest and (b) preparing a pharmacological agent comprising an amino acid sequence of the peptide selected in a handle region of an Fc domain. This process can be used to modify an Fc domain that is already linked through a term N or C or side chain to a peptide or a polypeptide (eg, etanercept). This procedure can also be used to modify an Fc domain that is part of an antibody (for example, adalimumab, eptratuzumab, infliximab, Herceptin (and the like). Thus, different molecules can be produced that have additional functionalities, such as a domain of binding to a different epitope or an additional binding domain to the existing epitope of the precursor molecule.The peptide can be selected, for example, by phage display, E. coli display, RNA-peptide ribosome display, base screening of yeast, chemical peptide screening, rational design or structural analysis of proteins. |