| abstract |
The present invention relates to: A method of cultivation of microorganisms of fungal origin, (preferably from the genera Aspergillus, Penicillium, Rhizopus, Trichoderma, Geotrichum, Fusarium, Mucor and Giberella), on a solid culture medium consisting of a solid support and a liquid containing the necessary nutrients for the culture, characterized by including the following stages: a) Crushing the support into particles, moistening it with the aqueous solution containing certain elements of the nutrient solution and sterilizing it; b) Impregnation of the support thus treated, with the nutrient solution containing the soluble carbon substratum or substrates, the growth agents and the inoculated material up to a humidity of between 50 and 85 percent as a whole. c) Fermentation of the medium; d) Extraction of 70 percent of the aqueous fraction containing the biosynthesized elements (secondary metabolites and / or enzymes), by compressing said solid support. |