abstract |
The invention relates to biotechnology, in particular to a process for cultivating cyanobacteria Spirulina platensis for obtaining biomass with a high content of phycobiliproteins. : NaNO3 - 2.5; NaHCO3 - 16.8; NaCl - 1.0; K2SO4 - 1.0; K2HPO4 · 3H2O - 1.0; MgSO4 · 7H2O - 0.20; CaCl2 · 6H2O - 0.04; H3BO3 - 0.00286; MnCl2 · 4H2O - 0.00181; ZnSO4 · 7H2O - 0.00022; CuSO4 · 5H2O - 0.00008; MoO3 - 0.000015, 1mL / L of 0.09 M FeHEDTA solution and distilled water up to 1 L. The first day of cultivation in the nutrient medium is added thiosemicarbaziddiacetethylenediamine Co (III) trihydrate of formula [Co (LH) En] · 3H2O, where: L - H2N-CS-NH-N (CH2COOH) and En - H2N-C2H4-NH2, in the amount of 10 ... 20 mg / L, at the same time the cultivation is performed in the light of 3000 ... 4500 lx and the temperature of 25 ... 30 ° C. |