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filingDate 2021-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7690a4e556c85178e57eaa505b6fef77
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publicationDate 2021-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber LU-500306-B1
titleOfInvention Method for detecting DNASE1L3 based on magnetic particle chemiluminescence immunoassay
abstract The invention relates to the technical field of immunological detection, in particular to a method for detecting DNASE1L3 based on magnetic particle chemiluminescence immunoassay. The method comprises the following steps: preparing biotinylated anti- DNASE1L3 antibody; preparing enzyme-labeled anti-DNASE1L3 antibody: combining the biotinylated anti-DNASE1L3 antibody with the anti-DNASE1L3 antibody in the sample to be detected; Adding streptavidin coated magnetic particles, washing under the action of magnetic force after reaction, and removing unbound substances; Adding enzyme-labeled anti-DNASE1L3 antibody, washing under the action of magnetic force after reaction, and removing unbound substances; Adding buffer solution and luminescent substrate solution for reaction, detecting luminescence intensity on luminometer, and calculating the concentration of DNASE1L3 in the sample. The method can quickly realize the quantitative analysis of DNASE1L3, and the preparation method and application of the tongue-and-groove structure gc3n4moo3 composite photocatalytic material have high detection sensitivity and strong specificity.
priorityDate 2020-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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